Long Term Enclosure Assessment on Stability of Soil Physical and Chemical Properties in Rangeland Ecosystems (Case Study: Khash County Rangelands)

Document Type : Research Paper

Authors

1 Rangeland and Watershed Management Department, Faculty of Water and Soil, University of Zabol, Zabol, Iran.

2 M.Sc. in Rangeland Management, Faculty of Water and Soil, University of Zabol, Zabol, Iran.

Abstract

This study aimed to investigate the effects of long-term (15-year) enclosure on the physical and chemical properties of soil in the arid and semi-arid rangelands of the Koteh region in Khash County. For this purpose, two sites were selected for comparison: an enclosed rangeland and a grazed rangeland with similar topography. Vegetation and soil samples were collected at two depths (0–15 cm and 15–30 cm), and parameters such as soil texture, pH, electrical conductivity, organic carbon, total nitrogen, phosphorus, potassium, and bulk density were measured. The results of data analysis showed that enclosure significantly increased canopy cover (32.3% vs. 10.9%), litter, and plant species diversity. At the 0–15 cm depth, organic carbon (0.48% vs. 0.24%) and total nitrogen (0.052% vs. 0.022%) were significantly higher in the enclosed area, while bulk density, available phosphorus, and potassium were higher in the grazed rangeland. At the 15–30 cm depth, organic carbon (0.33% vs. 0.23%) and total nitrogen were also higher in the enclosed area, with no observed differences in soil texture between the two sites. Additionally, pH and electrical conductivity values at both depths in the enclosed area were lower than or similar to those in the grazed rangeland, indicating a relative improvement in soil quality under long-term enclosure. These findings suggest that long-term enclosure can enhance the sustainability and ecological function of rangelands in arid and semi-arid environments by improving key soil indicators.




Introduction
Rangeland enclosure not only helps restore vegetation cover but also enhances the long-term sustainability and productivity of rangeland ecosystems by improving soil physical and chemical factors. Although ample evidence confirms the positive effects of enclosure, the results reported in some studies have been diverse and contradictory due to differences in climate, soil type, vegetation composition, and duration of enclosure. Considering the arid and semi-arid climatic conditions of the Koteh region in Khash County and the high vulnerability of its soils to overgrazing, a detailed study of the effects of long-term enclosure on the physical and chemical properties of the soil in this area is of great importance. Therefore, this study aims to scientifically assess the impact of enclosure on key soil indices in rangeland ecosystems in this region.
Materials and Methods
This study aimed to investigate the effect of long-term enclosure on the physical and chemical properties of soil in arid and semi-arid rangelands of Khash County. The Koteh rangeland was chosen as the enclosure site with a 15-year history of enclosure, and an adjacent rangeland with similar topographic conditions was chosen as the grazed site. Vegetation sampling was conducted in June by establishing six 100-meter transects at each site (three transects in the direction of the slope and three transects perpendicular to it) and sampling 10 one-meter plots in each transect using a random-systematic method. Data on canopy cover, litter, bare soil, and plant species were recorded. Soil sampling was performed from two depths of 0–15 and 15–30 cm at the beginning, middle, and end of the transects, and a total of 24 composite samples were prepared. Soil properties were measured, including texture, pH, electrical conductivity, total nitrogen, phosphorus, potassium, calcium, magnesium, sodium, organic carbon, and bulk density using standard methods. Data were analyzed using independent and paired t-tests in SPSS software. Correlation analysis was performed in R software.
Results and Discussion
The results showed that the vegetation of the area consisted of perennial and annual species with diverse growth forms. In the enclosure area, the canopy cover was significantly higher (32.3%) than in the grazed area (10.9%). More stable and diverse species were found in the enclosure area, and the amount of litter was also higher in this area (3.72%), indicating higher biological activity and reduced soil erosion. Regarding soil characteristics, the percentage of silt was higher in the grazed area, and the bulk density of the soil was also higher in the grazed area, indicating greater soil compaction due to livestock grazing. The amounts of organic carbon and total nitrogen at both depths (0–15 and 15–30 cm) in the enclosure area were significantly higher than in the grazed area, which could be due to the accumulation of plant residues, reduced erosion, increased biological activity, and improved microclimatic conditions. However, available phosphorus and potassium were higher in the grazed area. Also, soil acidity and electrical conductivity were lower in the enclosure area, indicating improved soil conditions. Soil texture composition was not significantly different at the depth of 15 to 30 cm, but bulk density and nutrients were still significantly different. These findings emphasize that livestock grazing management through enclosure improves vegetation cover and soil physical and chemical quality in semi-arid ecosystems.
Conclusion
According to the results of this study, long-term enclosure, as an effective management strategy, plays a significant role in restoring and improving the ecological conditions of arid and semi-arid rangelands. This method increases the percentage of vegetation cover and litter, preserves native and valuable rangeland species, and improves ecosystem dynamics. The decrease in soil bulk density indicates an improvement in the physical structure of the soil and a decrease in compaction caused by livestock grazing. From the perspective of chemical properties, a significant increase in organic carbon and total nitrogen, along with a decrease in acidity and electrical conductivity, indicates an improvement in soil quality and a strengthening of the nutrient cycle. Although the accumulation of phosphorus, potassium, and calcium in grazed areas was observed due to livestock waste, these elements are not stably stabilized and cannot replace the positive effects of enclosure. Therefore, it is recommended that enclosures be developed as a sustainable method in rangeland management and, along with continuous monitoring of soil and vegetation, help maintain and improve the health of the rangeland ecosystem.

Keywords

Main Subjects


Ai, M., Sun, Y., Yan, B., & Wei, Y. (2018). A summary of the impact of land degradation on soil carbon sequestration. IOP Conference Series: Materials Science and Engineering, 394, 052028. doi:10.1088/1757-899X/394/5/052028
Akbarzadeh, P., & Nikoo, S. (2022). Effects of Land Use Change on Groundwater Quality (Case Study: Damghan Watershed). Journal of Ecohydrology, 9(2), 437-459. doi: 10.22059/ije.2022.340711.1622. (In Persian).
Bagherian, R., Sefidi, K., Keivan Behjou, F., Soltani, A. A., & Behtari, B. (2018). The influence of changes in grazing intensity on soil properties in the southeastern alpine rangelands of Sabalan. Iranian Journal of Range and Desert Research, 25(1), 183-190. doi:10.22092/ijrdr.2018.116236
Bai, Y., & Cotrufo, M. F. (2022). Grassland soil carbon sequestration: Current understanding, challenges, and solutions. Science, 377, 603–608. doi: 10.1126/science.abo2380
Bardgett, R. D., & Wardle, D. A. (2010). Aboveground–belowground linkages: Biotic interactions, ecosystem processes, and global change. Oxford University Press. https://books.google.com/books
Bardgett, R. D., Bullock, J. M., Lavorel, S., Manning, P., Schaffner, U., Ostle, N., & Shi, H. (2021). Combatting global grassland degradation. Nature Reviews Earth & Environment, 2(10), 720-735. doi: 10.1038/s43017-021-00207-2
Barnard, J. H., Edeh, J. A., van Rensburg, L. D., & du Preez, C. C. (2025). Calibration of an apparent electrical conductivity sensor using capacitance probes for determining soil water content. Soil Science Society of America Journal, 89(1), e20799. doi: 10.1002/saj2.20799
Belteben, A. A., Gharaibeh, M. A., & Albalasmeh, A. A. (2020). Grazing effects on soil physical and chemical properties. Journal of Modern Urban and Agricultural Studies (JMUAS), 2, 29–47. doi: 10.36602/jmuas.2020.v02.01.03
Chen, L., Baoyin, T., & Xia, F. (2022). Grassland management strategies influence soil C, N, and P sequestration through shifting plant community composition in semi-arid grasslands of northern China. Ecological Indicators, 34, 1–12. doi: 10.1016/j.ecolind.2021.108470
Dashti, M., Mirdavoudi, H., Ghasemi Arian, A., & Azizi, N. (2021). Effects of Topography and Soil Variables on Abundance of Onobrychis chorassanica Bunge. in Kardeh and Kurtian Rangelands, Mashhad, Iran. Journal of Rangeland Science, 11(3), 283-299. https://journals.iau.ir/article_678922.html
Dastgheyb Shirazi, S. S., Ahmadi, A., Abdi, N., Toranj, H., & Khaleghi, M. R. (2021). Long-term grazing exclosure: implications on water erosion and soil physicochemical properties (case study: Bozdaghin rangelands, North Khorasan, Iran). Environmental Monitoring and Assessment, 193(1), 51. doi: 10.1007/s10661-020-08819-9
Ebrahimi, M., Khosravi, H., & Rigi, M. (2016). Short-term grazing exclusion from heavy livestock rangelands affects vegetation cover and soil properties in natural ecosystems of southeastern Iran. Ecological Engineering, 95, 10–18. doi: 10.1016/j.ecoleng.2016.06.069
Ghonchepour, M., Sadeghinia, M., Baghestani, N., Pourmirazee, A., & Kodouri, M. (2023). Comparison of vegetation and soil diversity inside and outside the enclosure (Case study: Artemisia habitats of Dehno, Bardsir, Kerman). Iranian Journal of Range and Desert Research, 30(1), 152-163. doi: 10.22092/ijrdr.2023.128952.
Heshmati, M., & Gheitury, M. (2021). Effects of Long Term Grazing Exclusion on Some Soil Physicochemical Characteristics and Sustainability. Iranian Journal of Soil Research, 35(1), 15-26. doi: 10.22092/ijsr.2021.352965.573. (In Persian).
Joneidi, H., Bazgir, E., & Kamali, N. (2025). Effectiveness of biological characteristics of rangeland soil in the face of land use change to dryland (Case study: Rangelands in Kermanshah). Journal of Environmental Erosion Research. 2025; 15 (1):25-44. doi: 10.61186/jeer.15.1.25. (In Persian).
Karimian, V., & Barani, H. (2025). Identification of Common Medicinal-Industrial Plants in Desert Rangelands and Examination of Local Community Awareness (Case Study: Khaf County, Khorasan Razavi Province). Desert Management, 12(4), 41-62. doi: 10.22034/jdmal.2025.2045459.1490. (In Persian).
Kaur, S., Chandel, S., Singh, K., Singh, D., & Singh, V. K. (2024). Comparative Assessment of Extractants for Available Potassium in Calcareous Soil of Punjab. Communications in Soil Science and Plant Analysis, 55(13), 2009-2027. doi: 10.1080/00103624.2024.2336577
Khatibi, R., & Farahi, M. (2024). Study on the Impact of Exclosures on the Physicochemical Properties of Rangeland Soil (Case Study: Northern Golestan Province, Sufikam Rangelands). Advanced Environmental Sciences, 22(3), 483-494. doi: 10.48308/envs.2024.1360. (In Persian).
Liu, L., Sayer, E. J., Deng, M., Li, P., Liu, W., Wang, X., & Piao, S. (2023). The grassland carbon cycle: Mechanisms, responses to global changes, and potential contribution to carbon neutrality. Fundamental Research, 3(2), 209-218. doi: 10.1016/j.fmre.2022.09.028
Liu, N., Kan, H. M., Yang, G. W., & Zhang, Y. J. (2015). Changes in plant, soil, and microbes in a typical steppe from simulated grazing: Explaining potential change in soil C. Ecological Monographs, 85, 269–286.   doi: 10.1890/14-1368.1
Moameri, M., Ghorbani, A., Dadjou, F., Biswas, A., & Varasteh, F. (2024). Soil and plant attributes under grazing exclusion in semi-steppic semi-arid rangelands, Iran. Arid Land Research and Management, 38(3), 343–361. doi: 10.1080/15324982.2023.2299017
Mohammadi-Samani, K., Joneidi Jafari, H., & Moradi, P. (2022). Variability of some chemical soil properties under different rangelands management. Iranian Journal of Range and Desert Research, 29(2), 126-130. doi: 10.22092/ijrdr.2022.126760. (In Persian).
Ng, W. K., Maxfield, P. J., Crew, A. P., Teixeira, D. L., Bevan, T., & Bell, M. J. (2025). Comparison of soil organic carbon measurement methods. Agronomy, 15(8), 1826. doi: 10.3390/agronomy15081826
Ng, W. K., Maxfield, P. J., Crew, A. P., Teixeira, D. L., Bevan, T., & Bell, M. J. (2025). Comparison of soil organic carbon measurement methods. Agronomy, 15(8), 1826. doi: 10.3390/agronomy15081826
Petermann, J. S., & Buzhdygan, O. Y. (2021). Grassland biodiversity. Current Biology, 31, R1195–R1201. doi: 10.1016/j.cub.2021.06.060
Qiu, L., Wei, X., Zhang, X., & Cheng, J. (2013). Ecosystem carbon and nitrogen accumulation after grazing exclusion in semiarid grassland. PLoS ONE, 8(1), e55433. doi: 10.1371/journal.pone.0055433
Qu, Q., Deng, L., Shangguan, Z., Sun, J., He, J., Wang, K., & Peñuelas, J. (2024). Belowground C sequestrations response to grazing exclusion in global grasslands: Dynamics and mechanisms. Agriculture, Ecosystems & Environment, 360, 108771. doi: 10.1016/j.agee.2023.108771
Qu, T. B., Du, W. C., Yuan, X., Yang, Z. M., Liu, D. B., Wang, D. L., & Yu, L. J. (2016). Impacts of grazing intensity and plant community composition on soil bacterial community diversity in a steppe grassland. PLoS ONE, 11(7), e0159680. doi: 10.1371/journal.pone.0159680
Qu, Y., Tang, J., Li, Z., Zhou, Z., Wang, J., Wang, S., & Cao, Y. (2020). Soil enzyme activity and microbial metabolic function diversity in soda saline–alkali rice paddy fields of northeast China. Sustainability, 12(23), 10095. doi: 10.3390/su122310095
Sharifi, J., & Akbarzadeh, M. (2016). Investigating the impact of exclosure on vegetation changes and restoration of rangeland utility indicator species in Ardabil Province. Journal of Rangeland, 4(10), 376–386. dor.isc.ac/dor/20.1001.1.20080891.1395.10.4.1.7. (In Persian).
Sheidai Karkaj, E., Rezaei, H., Niknahad Gharemakher, H., Jafari Footami, I., & Sharifian, A. (2019). The role of Exclosure in the change of aggregate stability and soil structure of rangelands in Golestan province. Iranian Journal of Range and Desert Research, 26(4), 904-917. doi: 10.22092/ijrdr.2019.120682. (In Persian).
Sheidaye Karkaj, E., Jafari Footami I., & Niknahad Gharmakher, H. 2017. The importance of climate in determining the effect of rangeland enclosure on change some soil characteristics of rangelands. Desert Ecosystem Engineering, 5(13): 39–56. https://deej.kashanu.ac.ir/article_112591_en. (In Persian).
Shyichuk, A., Ziółkowska, D., Lamkiewicz, J., & Kowalska, M. (2025). Calcium Determination by Complexometric Titration with Calcein Indicator Using Webcam for Endpoint Detection. Water, 17(12), 1757. doi: 10.3390/w17121757
Sollenberger, L. E., Kohmann, M. M., Dubeux, J. C. B., & Silveira, M. L. (2019). Grassland management affects delivery of regulating and supporting ecosystem services. Crop Science, 59, 441–459. doi: 10.2135/cropsci2018.09.0594
Tavousi, T., & Armesh, M. (2012). Statistical analysis and forecast of early frosts in Khash city during the statistical period of 1365–1387. Sepehr, 21(84), 28–30.
Tulloch, A. I., Healy, A., Silcock, J., Wardle, G. M., Dickman, C. R., Frank, A. S., ... & Greenville, A. C. (2023). Long‐term livestock exclusion increases plant richness and reproductive capacity in arid woodlands. Ecological Applications, 33(8), e2909. doi: 10.1002/eap.2909
Wang, X., Zhou, C., Zuo, S., Ji, Y., Liu, W., & Huang, D. (2024). Heavy grazing reduces soil bacterial diversity by increasing soil pH in a semi-arid steppe. PeerJ, 12, e17031. doi: 10.7717/peerj.17031
Wang, Y., Ju, X., Wu, Q., & Han, G. (2025). Effects of grazing intensity on microbial diversity at different soil depths in desert steppe soils. Agronomy, 15(1), 124. doi: 10.3390/agronomy15010124
Wang, Y., Lv, W., Xue, K., Wang, S., Zhang, L., Hu, R., ... & Niu, H. (2022). Grassland changes and adaptive management on the Qinghai–Tibetan Plateau. Nature Reviews Earth & Environment, 3(10), 668-683. doi: 10.1038/s43017-022-00330-8
Wu, X., Li, Z., Fu, B., Lu, F., Wang, D., Liu, H., & Liu, G. (2014). Effects of grazing exclusion on soil carbon and nitrogen storage in semi-arid grassland in Inner Mongolia, China. Chinese Geographical Science, 24(4), 479-487. doi: 10.1007/s11769-014-0694-1
Xu, H., You, C., Tan, B., Xu, L., Liu, Y., Wang, M., Xu, Z., Sardans, J., & Peñuelas, J. (2023). Effects of livestock grazing on the relationships between soil microbial community and soil carbon in grassland ecosystems. Science of The Total Environment, 881, 163416. doi: 10.1016/j.scitotenv.2023.163416
Xun, W., Yan, R., Ren, Y., Jin, D., Xiong, W., Zhang, G., Cui, Z., Xin, X., & Zhang, R. (2018). Grazing induced microbiome alterations drive soil organic carbon turnover and productivity in meadow steppe. Microbiome, 6, 170. doi: 10.1186/s40168-018-0544-y
Zhang, M., Delgado Baquerizo, M., Li, G., Isbell, F., Wang, Y., Hautier, Y., Wang, Y., Xiao, Y., Cai, J., Pan, X., & Wang, L. (2023). Experimental impacts of grazing on grassland biodiversity and function are explained by aridity. Nature Communications, 14, 5040. doi: 10.1038/s41467-023-40809-6
Zhou, S., Dong, Y., Yang, H., Yang, S., Julihaiti, A., Liu, Z., Li, H., Zhang, Y., & Wang, Y. (2024). Effects of grazing exclusion on soil properties, fungal community structure, and diversity in different grassland types. Ecology and Evolution, 14(3), e11056. doi: 10.1002/ece3.11056